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產(chǎn)品資料

RPMI 6666細(xì)胞

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產(chǎn)品名稱: RPMI 6666細(xì)胞
產(chǎn)品型號: RPMI 6666
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

RPMI 6666細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。RPMI 6666細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


RPMI 6666細(xì)胞  的詳細(xì)介紹

RPMI 6666細(xì)胞

細(xì)胞形態(tài): **樣

運(yùn)輸方式: 凍存運(yùn)輸

年限: 29 years

生長狀態(tài): 懸浮生長

ATCC Number: CCL-113?

器官來源: 外周血

相關(guān)**: **瘤

數(shù)量: 大量

是否是腫瘤細(xì)胞: 1

物種來源: 人

Designations: RPMI 6666

Depositors: GE Moore

RPMI 6666細(xì)胞Biosafety Level: 2 [Cell contain Herpesvirus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: RPMI 6666細(xì)胞lymphoblast


Source: Organ: peripheral blood

Disease: Hodgkin's disease; Hodgkin's lymphoma

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: HLA A2, A3, B7, B18

Age: 29 years

Gender: male

Ethnicity: Caucasian

Comments: EBNA positive.

Biological Safety CLASS II.

Propagation: RPMI 6666細(xì)胞ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days

Cultures can be maintained by the addition of fresh medium or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.

References: 22317: Moore GE, et al. Leukocyte cultures of patients with leukemia and lymphomas. N.Y. State J. Med. 66: 2757-2764, 1966. RPMI 6666細(xì)胞PubMed: 5232150

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