GCT細胞

運輸方式： 凍存運輸

年限： 29 years

ATCC Number： TIB-223?

相關**： 其他**

生長狀態： 貼壁生長

數量： 大量

是否是腫瘤細胞： 1

物種來源： 人

Designations: GCT [Giant Cell Tumor]

Depositors: J Brennan

GCT細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Disease: fibrous histiocytoma

Derived from metastatic site: lung

Cellular Products: colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator

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DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11,12

D16S539: 9

GCT細胞D5S818: 13,15

D7S820: 11,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,18

Age: 29 years

Gender: male

Comments: The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.

Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: GCT細胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks.

References: 1181: Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748

1182: DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234

1183: Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. GCT細胞Blood 58: 1148-1154, 1981. PubMed: 6975643

3550: Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979

58064: Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469