182-PF SK細(xì)胞

ATCC Number： CRL-1532?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 20 years

器官來(lái)源： 皮膚

182-PF SK細(xì)胞生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: 182-PF SK

Depositors: L Kopelovich

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: hereditary adenomatosis

Permits/Forms: 182-PF SK細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 20 years

Gender: male

Ethnicity: Caucasian

Comments: The tissue donor suffered from hereditary adenomatosis.

The line is capable of 10 population doublings after thawing.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: 182-PF SK細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.

References: 22736: Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781

22759: . . Prog. 182-PF SK細(xì)胞Cancer Res. Ther. 13: 97-108, 1980.