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產品資料

891.T NCI細胞

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產品名稱: 891.T NCI細胞
產品型號: 891.T NCI
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

891.T NCI細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環(huán)境、與品質良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。891.T NCI細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養(yǎng)基即可。


891.T NCI細胞  的詳細介紹

891.T NCI細胞

是否是腫瘤細胞: 1

物種來源: 人

年限: 21 years

ATCC Number: CRL-7629?

相關**: 其他**

運輸方式: 凍存運輸

細胞形態(tài): 成纖維樣

生長狀態(tài): 貼壁生長

數量: 大量

Designations: Hs 891.T

891.T NCI細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Disease: kidney carcinoma

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10, 13

D13S317: 12

D16S539: 11, 12

D5S818: 11

891.T NCI細胞D7S820: 10, 11

THO1: 6, 9

TPOX: 8, 9

vWA: 18

Cytogenetic Analysis: modal number = 46

Age: 21 years

Gender: female

Ethnicity: Caucasian

Comments: See also ATCC CRL-7621.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: 891.T NCI細胞Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37.0°C.


Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.

Medium renewal: Every 2 to 3 days

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002

Recommended serum: 891.T NCI細胞ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101

Phosphate-buffered saline: ATCC 30-2200

Cell culture tested DMSO: ATCC 4-X

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