MC/CAR細胞

ATCC Number： CRL-8083?

相關**： 多發(fā)性骨髓瘤

器官來源： 外周血

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運輸方式： 凍存運輸

細胞類型： B**細胞

是否是腫瘤細胞： 1

物種來源： 人

細胞形態(tài)： **樣

**類型： IgG1 (cytoplasmic); kappa light chain

年限： 81 years

MC/CAR細胞Designations: MC/CAR

Depositors: Research Corporation

Isotype: IgG1 (cytoplasmic); kappa light chain

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Disease: plasmacytoma; myeloma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. MC/CAR細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: complement (C3b)

Tumorigenic: Yes

Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,13

D16S539: 11,12

D5S818: 11,14

D7S820: 9,13

THO1: 6,9

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: normal male diploid

Isoenzymes: ADA, 1

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

MC/CAR細胞Age: 81 years

Gender: male

Ethnicity: Caucasian

Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]

The cells produce, but do not secrete, immunoglobulin.

No surface immunoglobulin.

8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.

Can be used as a human fusion line.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

43270: Drexler H, et al. MC/CAR細胞False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719