pET-11c(+)載體基本信息
別名: | pET-11c, pet 11c |
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質粒類型: | 大腸桿菌表達載體 |
表達水平: | 高 |
克隆方法: | 多克隆位點,限制性內切酶 |
載體大小: | 5672bp |
5' 測序引物: | T7 |
5' 測序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
載體標簽: | T7 tag (N-端) |
載體抗性: | Ampicillin |
備注: |
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
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產品目錄號: | 69438-3 |
穩定性: | 瞬時表達 Transient |
組成型: | 組成型 Constitutive |
病毒/非病毒: | 非病毒 |
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 316 | 188 | |
T7_Terminal_primer | 256 | 274 | |
T7_leader | 358 | 326 | |
Xpress_fwd_primer | 359 | 341 | |
T7_transl_en_RBS | 383 | 367 | |
lacO | 428 | 401 | |
T7_promoter | 446 | 428 | |
pBRrevBam_primer | 549 | 530 | |
lacI | 824 | 1915 | |
tet (576 - 1190) | 1974 | 2588 | |
ROP | 3227 | 3418 | |
pGEX_3_primer | 3434 | 3412 | |
Ampicillin | 5464 | 4604 | |
AmpR_promoter | 5534 | 5506 |
ORF | Start | End | |
---|---|---|---|
ORF frame 1 | 583 | 1083 | |
ORF frame 2 | 956 | 1915 | |
ORF frame 1 | 2393 | 1698 | |
ORF frame 3 | 1971 | 2588 | |
ORF frame 2 | 5464 | 4604 |
Enzyme Name | Cut |
---|---|
ClaI | 24 |
HindIII | 29 |
BamHI | 319 |
NheI | 350 |
NdeI | 357 |
XbaI | 395 |
BglII | 461 |
BclI | 1197 |
ApaI | 1394 |
HpaI | 1689 |
EagI | 2251 |
NruI | 2286 |
MscI | 2758 |
PstI | 4922 |
AatII | 5599 |
EcoRI | 5670 |
The pET-11a-d vectors carry an N-terminal T7?Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.