ATCC Number： HB-12317?

相關**： 其他**

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態： 貼壁生長

運輸方式： 凍存運輸

細胞形態： 其他

數量： 大量

器官來源： 大腦

NG108-15細胞Designations: NG108-15 [108CC15]

Depositors: Univ. Texas Southwestern Medical Cntr.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus (neuroblastoma); Rattus norvegicus (glioma) deposited as mouse (neuroblastoma); rat (glioma)

Morphology: flat; round; 10 to 100 micrometers diameter

Source: Organ: brain

Disease: glioblastoma; neuroblastoma

Cell Type: somatic cell hybrid

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Applications: transfection host NG108-15細胞(Roche Transfection Reagents)

Comments: The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. [112482]

The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. [51493]

Propagation: ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:

0.1 mM hypoxanthine (final conc.)

400 nM aminopterin (final conc.)

0.016 mM thymidine (final conc.)

10% fetal bovine serum (final conc.)

1.5 g/L sodium bicarbonate

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. NG108-15細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 51492: Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

51493: Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. NG108-15細胞PubMed: 2985920

112482: Bernd Hamprecht, personal communication