TR 310細(xì)胞

生長狀態(tài)： 懸浮生長

ATCC Number： HB-219?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

細(xì)胞形態(tài)： **樣

**類型： IgG2b

Designations: TR 310

TR 310細(xì)胞Depositors: IL Weissman

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: TR 310細(xì)胞immunoglobulin; monoclonal antibody; against mouse T cell receptor

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the Tk1 murine T cell lymphoma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

TR 310細(xì)胞The antibody reacts with a deteminant on the mouse T cell antigen receptor.

It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. TR 310細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.