GH1細胞

是否是腫瘤細胞： 0

物種來源： 大鼠

年限： 7 months

品系： Wistar-Furth

數量： 大量

器官來源： 垂體

運輸方式： 凍存運輸

細胞形態： 上皮樣

ATCC Number： CCL-82?

相關**： 腫瘤

生長狀態： 貼壁生長

GH1細胞Designations: GH1

Depositors: G Sato

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: pituitary

Strain: Wistar-Furth

Disease: tumor

Cellular Products: growth hormone; prolactin; somatotrophin

Permits/Forms: GH1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 1

Age: 7 months

Gender: female

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. GH1細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Cells attach in clusters. Cell will pile up and not become 100% confluent.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004

recommended serum: ATCC 30-2020

recommended serum: ATCC 30-2040

References: 879: Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399

881: Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281

22421: Takemoto H, et al. GH1細胞Adrenotropic activity of mammo-somatotropic tumors in rats and mice. I. Biologic aspects. Cancer Res. 22: 917-924, 1962. PubMed: 13984648