LTR228細胞

ATCC Number： HB-8502?

數量： 大量

相關**： 其他**

生長狀態： 懸浮生長

運輸方式： 凍存運輸

器官來源： 脾

年限： 5 years

細胞形態： **樣

**類型： IgM; kappa light chain

細胞類型： B**細胞

是否是腫瘤細胞： 0

LTR228細胞物種來源： 人

Designations: LTR228 [LTR 228-TG, derivative of WIL-2NS (ATCC CRL-8155)]

Depositors: Cetus Corp.

Isotype: IgM; kappa light chain

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: spleen

Disease: hereditary spherocytosis

Cell Type:LTR228細胞 B lymphoblast; Epstein-Barr virus (EBV) transformed

Cellular Products: immunoglobulin; monoclonal antibody (small amounts)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11

D16S539: 11,12

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,11

vWA: 17,20

Cytogenetic Analysis: modal number = 48; Y present

LTR228細胞Age: 5 years

Gender: male

Ethnicity: Caucasian

Comments: The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.

The cells have a high cloning efficiency in soft agar and by limiting dilution.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 0.03% L-glutamine and 0.02 mM 6-thioguanine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: fetal bovine serum, 95%; DMSO, 5%

Doubling Time: 16 hrs

Related Products: WIL2-NS, ATCC CRL-8155

TK6, ATCC CRL-8015

WIL2-S, ATCC CRL-8885

References: 3570: Raubitschek AR, et al. LTR228細胞Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986