Gekko lung-1細(xì)胞

ATCC Number： CCL-111?

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 其他

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

年限： *****

器官來(lái)源： 肺

細(xì)胞形態(tài)： 上皮樣

Designations: Gekko lung-1

Gekko lung-1細(xì)胞Depositors: HF Clark

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: lizard, gecko (Gekko gecko) deposited as lizard, gekko

Morphology: epithelial

Source: Organ: lung

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Isolation: Isolation date: June, 1965

Virus Susceptibility: Gekko lung-1細(xì)胞Vaccinia virus

Vesicular stomatitis, Orsay (Indiana)

Human poliovirus 1

Cytogenetic Analysis: modal number = 38; range = 34 to 135.

Bimodal stemline number of 37 and 38 chromosomes. Stable pseudodiploid karyotype in 38 chromosome stemline number. The change from normal is a constant deletion in one of the largest chromosomes. Slightly unstable karyology in 37 chromosome stemline number.

Age: *****

Gender: male

Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Temperature: 30.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Gekko lung-1細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 30?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Two to three times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22886: Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

26281: Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

26282: Cohen MM, Clark HF. Gekko lung-1細(xì)胞Spontaneous chromosomal alteraion in cell lines of poikilothermic origin (Gekko gecko). Cytogenetics 7: 16-26, 1968. PubMed: 5659169