GC-2spd(ts)細胞

細胞形態： 上皮樣

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態： 貼壁生長

數量： 大量

品系： BALB/c

運輸方式： 凍存運輸

年限： 6 weeks

ATCC Number： CRL-2196?

GC-2spd(ts)細胞Designations: GC-2spd(ts)

Depositors: MC Hofmann, J Millan

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Cell Type: spermatocyte; SV40 large T antigen transfected

Strain: BALB/c

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Age: 6 weeks

Gender: male

Comments: GC-2spd(ts) was established by stable cotransfection of freshly isolated spermatocytes with the SV40 large T antigen gene (pSV3neo, see ATCC 37150) and a temperature sensitive mutant of the p53 tumor suppressor gene (LTRp53cG9).

Cells were selected with G-418, cultivated for 6 months and single cell cloned three times by limiting dilution.

No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed.

The cells have lost their differentiation potential, and are currently arrested at a premeiotic stage.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

GC-2spd(ts)細胞Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:7 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22715: GC-2spd(ts)細胞Hofmann MC, et al. A haploid and a diploid cell coexist in an in vitro immortalized spermatogenic cell line. Dev. Genet. 16: 119-127, 1995. PubMed: 7736662

23344: Hofmann MC, et al. Immortalized germ cells undergo meiosis in vitro. Proc. Natl. Acad. Sci. USA 91: 5533-5537, 1994. PubMed: 8202522