GAP 8.3細(xì)胞
ATCC Number： HB-12?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
生長狀態(tài)： 懸浮生長
Designations: GAP 8.3
GAP 8.3細(xì)胞Depositors: AE Berger
Isotype: IgG2a; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GAP 8.3細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Mice were immunized with MOLT-4 cells a human T cell line (MOLT-4, ATCC CRL-1582).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes a 200000 dalton antigen analogous to the mouse T200 antigen and the rat common leukocyte antigen (it does not react with erythrocytes).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
GAP 8.3細(xì)胞Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 914: Berger AE, et al. GAP 8.3細(xì)胞A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769
