Gap Per細(xì)胞
細(xì)胞形態(tài)： 成纖維樣
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
數(shù)量： 大量
器官來(lái)源： 皮膚
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
ATCC Number： CRL-1335?
相關(guān)**： Ehlers-Danlos綜合征子
年限： 66 years
Gap Per細(xì)胞Designations: Gap Per
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Ehlers-Danlos syndrome, possible heterozygote
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.Gap Per細(xì)胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 66 years
Gender: male
Comments: The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandfather of Cor Per II (ATCC CRL-1243), No Per (ATCC CRL-1327) and Jo Per (ATCC CRL-1332).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Gap Per細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Gap Per細(xì)胞Subculture every 8 to 10 days.
