G355-5細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 貓
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： embryo
數(shù)量： 大量
器官來源： 大腦
ATCC Number： CRL-2033?
G355-5細(xì)胞相關(guān)**： 正常
細(xì)胞形態(tài)： 其他
Designations: G355-5
Depositors: KJ Dunn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Felis catus
Morphology: glial, astrocyte
Source: Organ: brain
Disease: normal
G355-5細(xì)胞Cell Type: astrocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: xenotropic murine leukemia virus; amphotropic murine leukemia virus; mink cell focus forming (MCF) virus; RD-114 virus; feline leukemia virus (FeLV) A, B, C; baboon endogenous virus (BaEV); macaque virus;
simian sarcoma associated virus (SSAV); feline immunodeficiency virus (34TF10)
Age: embryo
Comments: This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Propagation: G355-5細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
heat-inactivated fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution.
Remove the trypsin, add fresh trypsin (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
References: 22563: Haapala DK, et al. G355-5細(xì)胞Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093
22991: Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413
