G1B細(xì)胞
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
器官來源： 其他
是否是腫瘤細(xì)胞： 0
物種來源： 其他
數(shù)量： 大量
細(xì)胞形態(tài)： 其他
ATCC Number： CRL-2536?
年限： *****
Designations: G1B
G1B細(xì)胞Depositors: EJ Noga
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Clarias batrachus
Morphology: pleomorphic
Source: Organ: gill
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Comments: G1B細(xì)胞The G1B cell line was derived from normal gill tissue taken from a healthy ***** walking catfish. [49660]
The cells support the in vitro growth of Amyloodinium ocellatum, a dinoflagellate that infects the gills and skin of both marine and brackish water fishes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
24 mM HEPES
Temperature: 25.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin-0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. G1B細(xì)胞Allow the flask to sit at room temperature (or at 25.0°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.NOTE: Some batches of fetal bovine serum are toxic to these cells.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Preservation: culture medium 95%; DMSO, 5%
References: 49660: Noga EJ, Hartmann JX. Establishment of walking catfish (Clarias batrachus) cell lines and development of a channel catfish (Ictalurus punctatus) virus vaccine. Can. J. Fish. G1B細(xì)胞Aquat. Sci. 38: 925-930, 1981.
