G-8細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

組織來(lái)源： skeletal muscle

細(xì)胞形態(tài)： 成肌細(xì)胞

年限： fetus fetus

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

G-8細(xì)胞物種來(lái)源： 小鼠

ATCC Number： CRL-1456?

數(shù)量： 大量

Designations: G-8

Depositors: J Peacock

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

G-8細(xì)胞Morphology: myoblast

Source: Tissue: skeletal muscle

Cell Type: myoblast myoblast;

Cellular Products: myosin

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Receptors: acetylcholine (The cells are highly responsive to acetylcholine.)

Tumorigenic: No

G-8細(xì)胞Cytogenetic Analysis: modal number = 66

Age: fetus fetus

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Doubling Time: 25 to 27 hrs

References: 1063: Christian CN, et al. G-8細(xì)胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191