12MBr6細(xì)胞

器官來源： 肺

組織來源： bronchus

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-1576?

相關(guān)**： 正常

年限： 2 to 3 years

細(xì)胞類型： 上皮細(xì)胞

數(shù)量： 大量

12MBr6細(xì)胞是否是腫瘤細(xì)胞： 0

物種來源： 綠長尾猴

Designations: 12MBr6

Depositors: JL Caputo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: epithelial

Source: Organ: lung

Tissue: bronchus

Disease: normal

Cell Type: 12MBr6細(xì)胞epithelial

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Isolation: Isolation date: July, 1979

Applications: Epidermal growth factor (EGF) was used to initiate and maintain growth.

Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells.

12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey.

Receptors: epidermal growth factor (EGF)

Cytogenetic Analysis: modal number = 62; range = 43 to 64.

The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del(1)9 ?; M2 del(13) and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.

Age: 2 to 3 years

12MBr6細(xì)胞Gender: male

Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor (EGF) was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.

Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.

Subculturing: Protocol: .

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: 12MBr6細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.Add fresh medium, aspirate and dispense into new flasks using a minimum inoculum of 6 X 10(4) cells/sq cm.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times weekly

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020