LHR-1055細胞

運輸方式： 凍存運輸

ATCC Number： CRL-2687?

生長狀態： 懸浮生長

數量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態： **樣

**類型： IgG1; kappa light chain

LHR-1055細胞Designations: LHR-1055

Depositors: H Loosfelt, E Milgrom

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:LHR-1055細胞immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362).

The LHR-29 LHR-1055細胞(ATCC CRL-2685), LHR-74 (ATCC CRL-2686) and LHR-1055 (ATCC CRL-2687) antibodies are additive. They recognize different epitopes.

The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium LHR-1055細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 56152: Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186