1939-8G2細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-8134?

運輸方式： 凍存運輸

數量： 大量

生長狀態： 懸浮生長

細胞形態： **樣

1939-8G2細胞**類型： IgG1

Designations: 1939-8G2

Depositors: Univ. Texas System, Board of Regents

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:1939-8G2細胞 immunoglobulin; monoclonal antibody; against Treponema pallidum

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with T. pallidum.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody does not react with T. phagedenis.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. 1939-8G2細胞To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3539: Kettman JR, Norgard MV. 1939-8G2細胞Hybrid cell lines producing monoclonal antibodies directed against Treponema. US Patent 4,514,498 dated Apr 30 1985