SRE GFP Reporter Plasmid SRE-GFP報告基因質(zhì)粒
產(chǎn)品描述
SRE-GFP報告基因是自主研發(fā)的用于檢測SRF轉(zhuǎn)錄活性水平為目的的報告基因。三重復合體TCR/Elk1/SRF結(jié)合到SRE進行基因表達的調(diào)控。
SRE-GFP報告基因主要用于檢測細胞中MAPK/ERK信號通路、**研究、基因過表達和RNAi的表型分析等。
pGMSRE-GFP是改造后的哺乳動物真核表達載體，在其多克隆位點插入了多個SRE結(jié)合位點，可以高效地檢測SRE的激活水平。由于載體采用了GFP作為報告基因，更便于后續(xù)的檢測。同時，對載體中預測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點進行了適當?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得SRE-GFP報告基因更易于轉(zhuǎn)染。SRE GFP Reporter Plasmid SRE-GFP報告基因質(zhì)粒
質(zhì)粒圖譜
SRE GFP Reporter Plasmid SRE-GFP報告基因質(zhì)粒pGM SRE -GFP質(zhì)粒測序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運輸與保存方法
冰袋運輸。-20℃保存。
使用說明
pGMSRE-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動物細胞。
注意事項
1）本質(zhì)粒未經(jīng)翊圣生物允許不得用于任何商業(yè)用途，也不得移交給訂貨人實驗室以外的任何人或單位。
2）為了您的健康，實驗操作時請穿實驗服和帶一次性手套。
SRE GFP Reporter Plasmid SRE-GFP報告基因質(zhì)粒參考文獻
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[4] Hawkins JL, et al. Pharmacologic inhibition of site 1 protease activity inhibits sterol regulatory element-binding protein processing and reduces lipogenic enzyme gene expression and lipid synthesis in cultured cells and experimental animals. J Pharmacol Exp Ther. 326(3):801-8(2008).
[5] Aminova LR, et al. The C3 domain of Pasteurella multocida toxin is the minimal domain responsible for activation of Gq-dependent calcium and mitogenic signaling. Protein Sci. 17(5):945-9(2008). SRE GFP Reporter Plasmid SRE-GFP報告基因質(zhì)粒
